In-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens

Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradiol(EE2) as the precursor probe coupled with quantitative proteomics to identify protein targets of CEs and classify their binding strengths.儿茶酚雌激素（Catechol estrogens，CEs）是一类可与细胞蛋白质发生主动共价相互作用、进而影响分子功能的代谢性亲电试剂。目前尚无可行方法可在活体系统中鉴定其结合蛋白。本研究开发了一种基于点击化学（click chemistry）的分析策略：以炔雌醇（ethinylestradiol，EE2）作为前驱体探针，联用定量蛋白质组学（quantitative proteomics）技术，实现了CEs蛋白质靶点的鉴定及其结合强度的分类。