Absolute microbiota variation of Sesbania cannabina and sweet sorghum mixed silage

Elucidating the mechanism of the fiber conversion and CH4 mitigation underlying microbial metabolism is critical to improve protein-rich silage digestibility and preserve silage energy for ruminant nutrient absorption. However, few studies have combined the quantitative microbial function, via absolute quantification 16S rRNA sequencing (AQ-16S seq), and conversion products in silage to explain these mechanisms. Here, we build a workflow to detect the substrates and products of fiber conversion in Sesbania cannabina and sweet sorghum (SS) silage, and combined AQ-16S seq to reveal these mechanisms.

阐明微生物代谢介导的纤维转化与甲烷减排机制，对于提升高蛋白青贮饲料的消化率、留存青贮能量以保障反刍动物的营养吸收至关重要。然而，目前鲜有研究结合通过绝对定量16S rRNA测序（AQ-16S seq）获得的定量微生物功能与青贮中的转化产物，来阐释上述机制。本研究构建了一套可检测田菁与甜高粱（sweet sorghum，SS）青贮中纤维转化底物与产物的分析流程，并结合AQ-16S seq技术揭示了相关机制。