Knockdown of ASD risk genes leads to a shared transcriptional signature. Knockdown of ASD risk genes leads to a shared transcriptional signature

RNAseq analysis of shRNA-mediated knockdown of chromatin modifiers associated with Autism Spectrum Disorder Overall design: E16.5 primary cortical neurons were cultured for 5 days before infection by shRNAs targeting one of Ash1L, Chd8, Crebbp, Ehmt1, Nsd1, Gtf2i, or Luciferase. Neurons were cultured for 5 additional days before RNA was collected.

针对自闭症谱系障碍相关染色质调控因子的短发夹RNA（shRNA）介导敲低的RNA测序（RNAseq）分析。实验整体设计：将胚胎16.5天（E16.5）的原代皮层神经元体外培养5天后，使用靶向Ash1L、Chd8、Crebbp、Ehmt1、Nsd1、Gtf2i或荧光素酶（Luciferase）的shRNA进行感染。随后继续培养神经元5天，再收集RNA样本。