Regulation of human oocyte maturation through non-CG methylation

Oocyte maturation is a coordinated process that is tightly linked to reproductive potential. A better understanding of genome regulation during human oocyte maturation will not only answer an important question in biology, but also facilitate the development of in vitro maturation technology as a fertility treatment. We generated single-cell transcriptome and methylome data from human oocytes at different maturation stages to investigate how the genome is regulated during oocyte maturation, focusing on the potential regulatory role of non-CG methylation.

卵母细胞成熟是一项协同调控的精密过程，与生殖潜能紧密相关。深入解析人类卵母细胞成熟过程中的基因组调控机制，不仅可解答生物学领域的关键科学问题，还能推动体外成熟技术作为生育治疗手段的发展。本研究从不同成熟阶段的人类卵母细胞中获取了单细胞转录组（single-cell transcriptome）与甲基化组（methylome）数据，旨在探究卵母细胞成熟过程中的基因组调控模式，并重点聚焦非CG甲基化（non-CG methylation）的潜在调控功能。