Supporting data for: Effect of changing swarm's fastidious boundary value on environmental DNA clustering results
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Supporting material for the publication : Effect of changing swarm’s fastidious boundary value on environmental DNA clustering results (working title) by Milena Königshofen and Frédéric Mahé In this study, Königshofen & Mahé use three published metabarcoding SSU rRNA datasets (18S V4, 18S V9 and 16S V3V4 fasta files) to demonstrate the effect of swarm's fastidious option (https://github.com/torognes/swarm). Fasta files are derived from publicly available raw fastq files, following the pre-clustering pipeline available athttps://github.com/frederic-mahe/fred-metabarcoding-pipeline The three original publications are:- de Vargas et al. (2015): V9 eukaryotic data from the sunlit surface layer of the world’s open oceans (doi: 10.1126/science.1261605; bioproject: PRJEB6610),- Mahé et al. (2017): V4 eukaryotic data from surface soils of three lowland Neotropical rainforests (doi: 10.1038/s41559-017-0091; bioproject: PRJNA317860),- Alonso et al. (2020): V3V4 bacterial data from plants stems collected in Chinese rice terraces (doi: 10.1111/1462-2920.15114; bioproject: PRJNA573048).
本研究配套发表材料: 《调整swarm严谨边界值对环境DNA聚类结果的影响》(暂定标题) 作者:Milena Königshofen与Frédéric Mahé 本研究中,Königshofen与Mahé使用三套已发表的元条形码(metabarcoding)小亚基核糖体RNA(SSU rRNA)数据集(18S V4、18S V9及16S V3V4的FASTA文件),以验证swarm严谨性选项的作用效果,相关工具的开源地址为https://github.com/torognes/swarm。 本研究所用的FASTA文件均源自公开可用的原始FASTQ文件,通过https://github.com/frederic-mahe/fred-metabarcoding-pipeline提供的预聚类流程处理得到。 本研究依托的三篇原始文献如下: - de Vargas等人(2015):来自全球开阔海洋透光表层的V9区真核生物数据(DOI: 10.1126/science.1261605;生物项目编号:PRJEB6610) - Mahé等人(2017):来自三处新热带低地热带雨林表层土壤的V4区真核生物数据(DOI: 10.1038/s41559-017-0091;生物项目编号:PRJNA317860) - Alonso等人(2020):取自中国水稻梯田植物茎秆的V3V4区细菌数据(DOI: 10.1111/1462-2920.15114;生物项目编号:PRJNA573048)



