Anigozanthos manglesii sequence reads. Anigozanthos manglesii

Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the Red and Green Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae). Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was then used to identify 190 000 microsatellite containing regions and priming sites. Fifteen polymorphic markers were then developed in the laboratory. These markers will be used in direct comparisons of the impact of bird and insect pollination on A manglesii, and provide a resource for population genetic studies in other Anigozanthos species.

本研究开发微卫星标记（Microsatellites），用于未来评估红绿袋鼠爪（Anigozanthos manglesii，血草科Haemodoraceae）的种群遗传结构、交配系统与种群扩散模式。研究采用个人基因组测序仪（Personal Genome Machine, PGM）半导体测序平台，生成约403万条序列读段；随后借助QDD流程软件，筛选得到19万个包含微卫星位点及引物结合区域的序列。实验室后续开发出15个多态性分子标记，此类标记既可用于直接比较鸟类与昆虫传粉对A. manglesii的影响，也可为其他袋鼠爪属（Anigozanthos）物种的种群遗传研究提供可用资源。