Effect of melsmon on NHDF cells under oxidative stress. Homo sapiens

Oxidative-stressed NHDF cells treated with or without Melsmon HPE at 500ug/ml for 72 hours. Cells were collected by scraping and RNA was extracted using Qiagen RNAeasy Plus Mini Kit. Libraries were prepared using AmpliSeq cDNA Synthesis for Illumina Kit, AmpliSeq for Illumina Transcriptome Human Gene Expression Panel, AmpliSeq Library PLUS for Illumina kit as per manufacturer's instruction. Sequencing was carried out using the Illumina MiSeq system and MiSeq Reagent Kit v3 (600-cycle).

以500μg/mL浓度的Melsmon HPE处理或不处理氧化应激状态的正常人真皮成纤维细胞（Normal Human Dermal Fibroblasts, NHDF），处理时长为72小时。通过细胞刮取法收集细胞，并使用凯杰（Qiagen）RNAeasy Plus Mini Kit提取总RNA；随后按照试剂盒制造商的操作说明，使用AmpliSeq cDNA Synthesis for Illumina Kit、AmpliSeq for Illumina Transcriptome Human Gene Expression Panel及AmpliSeq Library PLUS for Illumina Kit制备测序文库；最终采用因美纳（Illumina）MiSeq测序系统及MiSeq Reagent Kit v3（600循环）完成测序。