Vitis vinifera embryogenic callus epigenomics (RNA-seq)

Plants regenerated from tissue culture frequently show somaclonal variation. In this study we compared the transcriptomic and epigenetic state of embryogenic callus of grapevine with leaves from mature grapevine plants. In particular, we focussed on the expression of transposable elements and changes in siRNA abundance and genome-wide methylation in these tissues. Overall design: Comparison of gene and transposable element transcription, small RNA and DNA methylation in embryogenic callus cells with leaf tissue

通过组织培养再生的植株常表现出体细胞无性系变异（somaclonal variation）。本研究对比了葡萄胚性愈伤组织（embryogenic callus）与成熟葡萄植株叶片的转录组学及表观遗传学状态。具体而言，本研究聚焦于这两类组织中转座因子（transposable elements）的表达情况、小干扰RNA（siRNA）丰度变化以及全基因组甲基化水平。实验整体设计：对比胚性愈伤组织细胞与叶片组织中基因及转座因子的转录水平、小RNA以及DNA甲基化状态。