Whole genome bisulfite sequencing of rumen, muscle, placenta, sperm tissues in cattle

In this study, we generated whole genome bisulfite sequencing data 4 tissues in cattle. We analyzed the variations of DNA methylation among tissues. Genomic DNA for each tissue was isolated according to the QIAamp DNA Mini Kit protocol (QIAGEN, Valencia, CA, USA). The quality of DNA samples was evaluated using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) including degradation, and potential RNA contamination, and purity (OD260/OD280), and concentration using a spectrophotometer (NanoDrop Technologies, Rockland, DE) to meet the requirements for library construction. The qualified genomic DNA from somatic tissues and sperm were used to construct a library. The library different lanes of a HiSeq X Ten (Illumina, San Diego, CA, USA) to generate 150-bp paired-end reads by Novogene (Novogene, Beijing, China).

本研究生成了牛4种组织的全基因组亚硫酸氢盐测序（whole genome bisulfite sequencing）数据，并对不同组织间的DNA甲基化变异情况开展分析。本研究依据QIAGEN公司（美国加利福尼亚州巴伦西亚）的QIAamp DNA Mini Kit试剂盒操作规范，提取各组织的基因组DNA。采用2100生物分析仪（2100 Bioanalyzer，安捷伦科技公司，美国加利福尼亚州圣克拉拉）对DNA样品的降解程度、潜在RNA污染情况进行检测，并通过分光光度计（NanoDrop Technologies，美国特拉华州罗克兰）测定样品的纯度（OD260/OD280比值）与浓度，确保其满足文库构建的质控要求。将符合质控标准的体细胞组织与精子基因组DNA用于文库构建，随后由诺禾致源（Novogene，中国北京）在Illumina公司（美国加利福尼亚州圣迭戈）的HiSeq X Ten测序平台的不同泳道上完成测序，最终产出150 bp双端读段。