Structure of metalloproteins, membrane proteins, filamentous sarcomer proteins, and virus particles

Our work focusses on biological nitrogen fixation by Nitrogenase, on metalloenzymes and integral membrane proteins of bacterial and archaeal respiratory systems (Andrade/Einsle/Wohlwend), the working mechanism and regulation of medically relevant membrane proteins and complexes and their defects leading to pathologies (Hunte), bacterial antibiotic resistance factors and viral and bacterial proteins bound to their targets or inhibitors, and their interactions with glycans (Stehle), human S100 proteins involved in nutritional immunity, USP18 enforcing the endogenous antiviral interferon response, and the respiratory complex NQR of V. cholerae (Fritz), LDL lipoprotein particles with low resolution data collection and phasing to find envelopes of parts of membrane proteins escaping conventional methods (Baumstark/Lunin), and on multi-domain components of the sarcomeric filamentous protein titin and their binding to the E3 ubiquitin ligase MuRF1 and the central myosin rod-domain (Mayans).

本研究聚焦于固氮酶（Nitrogenase）催化的生物固氮，以及细菌与古菌呼吸体系统中的金属酶与整合膜蛋白（integral membrane proteins）（Andrade、Einsle与Wohlwend课题组）；其次涵盖医学相关膜蛋白及复合物的工作机制与调控规律，及其功能缺陷引发的病理表型（Hunte课题组）；此外包含细菌抗生素耐药因子、与靶点或抑制剂结合的病毒及细菌蛋白，及其与聚糖（glycans）的相互作用（Stehle课题组）；还涉及参与营养免疫的人源S100蛋白、介导内源性抗病毒干扰素应答的USP18，以及霍乱弧菌（V. cholerae）的呼吸复合物NQR（Fritz课题组）；同时包含通过低分辨率数据采集与相位解析，解析常规方法难以解析的膜蛋白部分区域包膜结构的低密度脂蛋白（LDL）颗粒研究（Baumstark与Lunin课题组）；最后聚焦于肌节丝状蛋白肌联蛋白（titin）的多结构域组分，及其与E3泛素连接酶MuRF1和肌球蛋白核心杆状结构域的结合相互作用（Mayans课题组）。