COS7 tfx pNL4GFP and VPS4B(E235Q)
收藏Mendeley Data2024-01-31 更新2024-06-29 收录
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https://data.caltech.edu/doi/10.22002/t4p4v-x8x17
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资源简介：
Tilt Series Date: 2012-03-15 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: Raptor Collaborators and Roles: VPS4B (E235Q) expression construct provided by W.Sundquist (Utah); pNLGFP construct provided by I. Chen (UCLA) Purification / Growth Conditions / Treatment: lipofectamine transfection Sample Preparation: manual blotting
倾斜序列日期：2012-03-15
数据采集者：Cora Woodward
物种/标本：非洲绿猴（Cercopithecus aethiops，African green monkey）
细胞株：COS-7 ATCC CRL-1651
倾斜序列设置：单轴倾斜模式，倾斜范围为(-50.0°, 50.0°)，步长1.5°，采用恒定角增量，辐照剂量60.0 eV/Ų，散焦值-6.0 μm，放大倍数22500×
采集软件：UCSF tomo
上传方式：rundir
所用处理软件：Raptor
合作者及分工：犹他大学W.Sundquist提供VPS4B (E235Q) 表达载体；加州大学洛杉矶分校I. Chen提供pNLGFP载体
纯化/培养条件与处理：脂质体转染（lipofectamine transfection）
样品制备：手工印迹
创建时间：
2024-01-31
