Transcriptome profiling of WT and BS lymphoblast and fibroblast human cell lines, WT and BLM knock-out mouse ES cells from 129/CAST F1 hybrids

We performed RNA-seq experiments to compare effect of Bloom Syndrome / Blm knock-out on gene expression. The following cell lines were used: GM12982 (human lymphoblasts, WT), GM16375, GM17361 (human lymphoblasts, BS); GM07492, GM07545 (human fibroblasts, WT), GM02085, GM03402 (human fibroblasts, BS), mouse ES cells from F1 cross between strains 129 and CAST: WT and with Blm knocked-out. The experiments were done in triplicates.

本研究开展RNA测序（RNA-seq）实验，旨在对比分析布卢姆综合征（Bloom Syndrome）相关Blm基因敲除对基因表达的影响。本次实验所用细胞系如下：GM12982（人淋巴母细胞，野生型（Wild Type，简称WT））、GM16375、GM17361（人淋巴母细胞，BS型（即布卢姆综合征））；GM07492、GM07545（人成纤维细胞，野生型）、GM02085、GM03402（人成纤维细胞，BS型）；以及由129与CAST品系经F1杂交得到的小鼠胚胎干细胞，包含野生型与Blm基因敲除型两种组别。所有实验均设置三次生物学重复。