Characterization of dairy herds Girolando through typification of molecular markers for kappa-caseína

This study aimed to characterize the genotype of kappa-casein in 144 Gir cows, the State of Pernambuco, using PCR-RFLP. 30 ½ Holstein-gir-gir 38 ¾ Holstein and Holstein-turned 76 ⅝: Whole blood samples of the animals of the following genotypes were collected. This material was extracted DNA. Through a region of DNA was amplified and its product has the action of restriction enzyme HindIII possible to observe the polymorphism was determined. The following genotypes with respective frequencies were found: AA (0.59); AB (0.25) BB (0.06); CA (0.05); BC (0.02) and CC (0.02). The allele frequencies observed for genes A, B and C were 0.74; 0.20 and 0.06, respectively. Important to note the detection of the C gene in the population, rarely described in other papers. The presence of the BB genotype in the population of Holstein cows-turned points for deployment of genetic selection for increased frequency of the genotype in question, which will enable significant gains in the milk chain, particularly for producers and processing industries programs.

本研究旨在利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术，对巴西伯南布哥州144头吉尔牛（Gir）的κ-酪蛋白（kappa-casein）基因型进行分型鉴定。本研究共采集三类牛只的全血样本：30头携带1/2荷斯坦血统的荷斯坦-吉尔混血牛、38头携带3/4荷斯坦血统的牛，以及76头携带7/8荷斯坦血统的改良荷斯坦牛，并从上述样本中提取基因组DNA。通过扩增一段靶DNA区域，并借助限制性内切酶HindIII酶切扩增产物，完成多态性分型鉴定。最终检出以下κ-酪蛋白基因型及其对应频率：AA型（0.59）、AB型（0.25）、BB型（0.06）、CA型（0.05）、BC型（0.02）及CC型（0.02）。A、B、C三个等位基因的频率分别为0.74、0.20及0.06。值得注意的是，本研究在该牛群中检出了C等位基因，此类报道在既往文献中较为罕见。该荷斯坦牛群中BB基因型的存在，为开展遗传选育以提升该目标基因型的频率提供了可行基础，此举将为乳业产业链带来显著效益，尤其有助于养殖户与加工企业的相关项目推进。