Host genetics influences gut microbiota over generations in pigs. Enterotype G0-G3

We carried out a study to demonstrate the impact of the host genetics on gut microbiota established one month post-weaning, by directional selection two pig lines over three generations (G0 to G3). The gut microbiota was characterized by sequencing the V3-V4 variable region of the 16S rRNA gene from faecal samples collected on 60-day-old Large White piglets. A subset of samples from the G0 generation were analysed with a whole-metagenome sequencing approach.

本研究通过对两个猪品系开展为期三代（G0至G3）的定向选育，旨在阐明宿主遗传学对断奶后一个月建立的肠道菌群的影响。研究以60日龄大白猪仔猪的粪便样本为材料，通过对其中16S rRNA基因的V3-V4可变区进行测序，完成肠道菌群的表征分析；同时针对G0代的部分样本，采用全宏基因组测序（whole-metagenome sequencing）方法开展分析。