有/无葡萄糖培养的MDSC转录组芯片

纯化的CD34+细胞通过造血干细胞扩增培养基培养7-8天后，在有或者无葡萄糖的条件下进行G-CSF、GM-CSF刺激后，使用TRIZOL法提取总RNA。通过Agilent Bioanalyzer 2100检测RNA质量后，进一步用RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany)进行纯化。在合作公司通过Agilent-014850 Whole Human Genome Microarray 4x44K G4112F转录组芯片分析样本的转录组数据，并进行数据处理和差异基因分析，得到的数据样本通过实时荧光定量聚合酶链锁反应（RT-PCR）验证。Purified CD34+ cells were cultured in hematopoietic stem cell expansion medium for 7–8 days, then stimulated with G-CSF and GM-CSF under glucose-supplemented or glucose-free conditions. Total RNA was extracted using the TRIZOL method, followed by RNA quality assessment with the Agilent Bioanalyzer 2100. The RNA was further purified using the RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). Transcriptomic profiling of the samples was conducted at the collaborating company using the Agilent-014850 Whole Human Genome Microarray 4x44K G4112F, with subsequent data processing and differential gene expression analysis performed. The resulting transcriptomic dataset was validated via quantitative real-time polymerase chain reaction (RT-PCR).

搜集汇总

背景与挑战

背景概述

该数据集记录了人CD34+造血干前体细胞经G-CSF和GM-CSF诱导分化为髓系抑制性细胞（MDSC）后，在有或无葡萄糖培养条件下的转录组表达谱。通过Agilent全基因组芯片进行差异基因分析，并用RT-PCR验证，旨在探究葡萄糖对MDSC基因表达的影响。