UDiTaS™, a genome editing detection method for indels and genome rearrangements

We have developed a Uni-Directional Targeted Sequencing methodology, UDiTaS, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion events (indels), all in a single reaction.我们研发了一种命名为UDiTaS的单向靶向测序（Uni-Directional Targeted Sequencing）方法，该方法具备定量特性，可消除可变长度聚合酶链式反应（PCR）扩增所带来的偏倚，且能够在单次反应中同时检测小插入缺失事件（indels）与结构变异。