ATAC-seq, histone-seq, and RNA-seq of mouse craniofacial tissues [RNA-seq]

To investigate the role of the transcription factor AP-2 in craniofacial development, we performed: ATAC-seq on E11.5 craniofacial surface ectoderm in control and Tfap2a/Tfap2b ectoderm knock-out embryos; RNA-seq on E10.5 craniofacial prominences from control and Tfap2a/Tfap2b ectoderm knock-out embryos; histone-seq (H3K4me3) in E10.5 and E11.5 wild-type craniofacial surface ectoderm. To investigate the role of the transcription factor AP-2 in craniofacial development, we performed: ATAC-seq on E11.5 craniofacial surface ectoderm in control and Tfap2a/Tfap2b ectoderm knock-out embryos; RNA-seq on E10.5 craniofacial prominences from control and Tfap2a/Tfap2b ectoderm knock-out embryos; histone-seq (H3K4me3) in E10.5 and E11.5 wild-type craniofacial surface ectoderm.为探究转录因子AP-2（transcription factor AP-2）在颅面发育中的作用，我们开展了如下实验：对对照组及Tfap2a/Tfap2b外胚层敲除胚胎的E11.5颅面表面外胚层进行ATAC-seq检测；对对照组与Tfap2a/Tfap2b外胚层敲除胚胎的E10.5颅面突起进行RNA-seq检测；对E10.5及E11.5野生型颅面表面外胚层进行组蛋白测序（H3K4me3）。为探究转录因子AP-2在颅面发育中的作用，我们开展了如下实验：对对照组及Tfap2a/Tfap2b外胚层敲除胚胎的E11.5颅面表面外胚层进行ATAC-seq检测；对对照组与Tfap2a/Tfap2b外胚层敲除胚胎的E10.5颅面突起进行RNA-seq检测；对E10.5及E11.5野生型颅面表面外胚层进行组蛋白测序（H3K4me3）。