Gene expression in E9.5 hearts comparing wt with Arid3b-null embryos

We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Hearts were dissected from E9.5 embryos (including the heart tube and the pharyngeal mesoderm and endoderm located beneath). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.我们对E9.5期心脏组织中的野生型（wild-type）胚胎与Arid3b缺陷型胚胎的mRNA表达水平开展了分析与比较，旨在鉴定差异表达基因，为阐明Arid3b在胚胎发育过程中的功能提供线索。研究人员从E9.5期胚胎中解剖分离心脏组织，该组织包含心管及其下方的咽中胚层（pharyngeal mesoderm）与内胚层（endoderm）。针对每一轮基因芯片（microarray）三次重复实验，我们均收集4枚野生型胚胎与4枚Arid3b缺陷型胚胎并分别混合，以制备实验样本。