Transcriptome analysis of cardiac specific depletion of RBPMS and RBPMS2 embryonic hearts [sch4_rnaseq]

To investigate the function of RNA-binding protein with multiple splicing (RBPMS) family, RBPMS and RBPMS2, in heart, we establish two RBPMS/2 double knock out mutant strains using cardiomyocyte specific Cre delete strains. Xenopus laevis light chain 2 (XMLC2) promoter CRE mice (Breckenridge et al., 2007) were crossed to RBPMSflox/flox / RBPMS2flox/flox animals to generate the RBPMS/2flox/flox / XMLC2-Cre mice line. In a parallel approach, we crossbred RBPMSflox/flox / RBPMS2flox/flox mice with animals carrying alpha myosin-heavy chain (Myh6) Cre (MyHC-Cre) (Agah et al., 1997), resulting in the RBPMS/2flox/flox / aMyHC-Cre line. RNA seq of E11.5 (XML-Cre) and E16.5 (MyHC-Cre) embryonic hearts was performed. Comparative gene expression profiling analysis of RNA-seq data for littermate control and cardiomyocyte specific double knock out of RBPMS/2 hearts at embryonic stage.为探究多剪接RNA结合蛋白（RNA-binding protein with multiple splicing, RBPMS）家族成员RBPMS与RBPMS2在心脏中的功能，我们借助心肌细胞特异性Cre重组酶敲除工具鼠，构建了两种RBPMS/2双基因条件性敲除突变品系。将非洲爪蟾轻链2（Xenopus laevis light chain 2, XMLC2）启动子驱动的Cre重组酶工具鼠（Breckenridge等，2007）与RBPMS基因flox纯合/RBPMS2基因flox纯合的小鼠交配，获得RBPMS/2基因flox纯合/XMLC2-Cre品系小鼠。平行实验中，我们将RBPMS基因flox纯合/RBPMS2基因flox纯合的小鼠与携带α肌球蛋白重链（alpha myosin-heavy chain, Myh6）Cre重组酶（MyHC-Cre）的小鼠交配（Agah等，1997），得到RBPMS/2基因flox纯合/aMyHC-Cre品系。随后分别对胚胎发育第11.5天（XML-Cre组）与胚胎发育第16.5天（MyHC-Cre组）的胚胎心脏进行RNA测序（RNA-seq），并针对胚胎时期同窝对照小鼠与心肌细胞特异性RBPMS/2双敲除小鼠的心脏RNA-seq数据开展比较基因表达谱分析。