Transcription profiling of mouse PERK mutant vs. wild type

Molecular characterization of PERK's role in the mouse exocrine pancreas. Postnatal days 16, 19 and 32 were examined. Postnatal day 16 represents a stage immediately prior to severe cytological changes in the exPKO mice. Postnatal day 19 represents the initial stage of severe phenotypes in the exPKO mice. Postnatal day 32 represents the middle stage of severe phenotypes (about 2 wks from the initiation) in the exPKO mice. Experiment Overall Design: Comparison between the two genotypes (Perk mutant vs. wild-type) at postnatal days 16, 19 and 32.本数据集针对小鼠外分泌胰腺中蛋白激酶R样内质网激酶（PERK）的功能开展分子表征研究。实验选取生后第16、19和32天三个时间点进行检测：生后第16天对应exPKO小鼠出现严重细胞学变化前的即刻阶段；生后第19天对应exPKO小鼠严重表型的初始阶段；生后第32天对应exPKO小鼠严重表型的中期阶段（即表型起始后约2周时）。实验整体设计为：在生后第16、19和32天，对两种基因型（Perk突变型与野生型）进行对比分析。