微环境控释型导电水凝胶治疗脊髓损伤

本研究设计了一种MMP响应的明胶基导电水凝胶（GCH），通过控制明胶和PPy浓度，匹配脊髓力学性能和电导率。为了实现响应释放，对GCH修饰谷胱甘肽（GSH），与重组蛋白GST-TIMP-bFGF结合后制备出MMP响应的bFGF导电水凝胶。将其移植入大鼠SCI模型，发现MMP响应的导电水凝胶可在体内改善神经再生微环境，促进神经组织修复及功能恢复。在研究过程中，分别设计了细胞层面实验和动物损伤实验，使用雌性SD大鼠作为动物模型开展损伤修复模型，在分组中设计了假手术组对照、空白对照、不导电水凝胶对照、不导电因子对照，对所获取的实验数据使用Graphpad prism 7.0软件进行统计分析和绘制统计图，所有数据采用平均值 ± 标准差的形式表示。采用Student’s t-test检验方法比较两组间的计量数据；采用One-way ANOVA test检验方法比较多组间的计量数据，组内两两多重比较采用LSD-t检验方法。科学数据的获取和统计分析全面规范，为研究方向的进一步深入和应用提供了研究基础。

This study designed an MMP-responsive gelatin-based conductive hydrogel (GCH) by controlling the concentrations of gelatin and polypyrrole (PPy) to match the mechanical properties and conductivity of spinal cord tissue. To achieve stimulus-responsive release, the GCH was modified with glutathione (GSH), and combined with recombinant protein GST-TIMP-bFGF to prepare an MMP-responsive bFGF-loaded conductive hydrogel. When transplanted into a rat spinal cord injury (SCI) model, this MMP-responsive conductive hydrogel was found to improve the neural regeneration microenvironment in vivo, and promote neural tissue repair and functional recovery. During the study, cell-level experiments and animal injury experiments were conducted. Female Sprague-Dawley (SD) rats were used as the animal model to establish the injury repair model. The experimental groups included a sham operation group control, blank control, non-conductive hydrogel control, and non-conductive factor control. Statistical analysis and graph plotting were performed using GraphPad Prism 7.0 software. All data are presented as mean ± standard deviation (SD). Student’s t-test was used to compare quantitative data between two groups; one-way ANOVA was employed for comparison among multiple groups, with LSD-t test adopted for pairwise multiple comparisons within groups. The comprehensive and standardized acquisition and statistical analysis of the scientific data provide a solid research foundation for further in-depth studies and applications of this research direction.