SCF -licensed induction of a mucosal mast cell-like transcriptional program by TGF-b

This study evaluates the effects of 72h stimulus with stem cell factor (SCF) (100 ng/mL) and TGF-b (1 ng/mL or 10 ng/mL), both alone and in combination, on the transcriptome of bone marrow-derived cultured mast cells (BMMCs) grown in IL-3 for 6-8 weeks. Through this approach, we find that SCF dramatically enhances TGF-b mediated upregulation of signature genes associated with mucosal mast cells, including transcripts encoding the proteases mMCP-1 (Mcpt1) and mMCP-2 (Mcpt2). Replicates indicate biologic duplicates. Samples were sequenced on an Illumina NextSeq 500.本研究针对以白细胞介素3（interleukin 3, IL-3）培养6~8周的骨髓源性培养肥大细胞（bone marrow-derived cultured mast cells, BMMCs），评估了单独或联合施加干细胞因子（stem cell factor, SCF，100 ng/mL）与转化生长因子β（transforming growth factor β, TGF-β，1 ng/mL或10 ng/mL）进行72小时刺激对其转录组的影响。通过该实验策略，我们发现干细胞因子可显著增强转化生长因子β介导的黏膜肥大细胞特征基因上调，包括编码蛋白酶mMCP-1（Mcpt1）与mMCP-2（Mcpt2）的转录本。本研究的重复样本均为生物学重复。所有样本均在Illumina NextSeq 500测序平台上完成测序。