Pharmacological inhibition of the IKK complex inhibits IL-6 production in immature H4 cells.

Immature H4 cells (A) and mature NCM-460 cells (B) were plated in 24 well plates overnight, incubated in low serum media for 3h and treated with IL-1β (0.5ng/ml) in the absence or presence of the IKK complex inhibitor wedelolactone at the indicated concentrations (triplicate treatments) as indicated. Tissue culture media was harvested after 6 h and assayed for IL-6 by ELISA. Mean +/-S.E (n = 3) from 2 experiments are presented, control un induced compared to IL-1 induced and IL-1 induced compared to IL-1 in the presence of the inhibitors, analyzed by Student T-test. (PDF)