Stress-induced Metabolic Remodeling of Adipose and Brain Tissue Revealed by Positron Emission Tomography

Overview This repository contains the figure source data, raw data, and code associated with the manuscript: “Stress-induced Metabolic Remodeling of Adipose and Brain Tissue Revealed by Positron Emission Tomography” Preprint DOI: https://doi.org/10.64898/2026.09.09.749803. Publication DOI: Pending (Under Revision at Communications Biology: COMMSBIO-26-4474A) Contact: Chrysoula Vraka ([e-mail]), Division of Nuclear Medicine, Medical University of Vienna, Austria. Citation: When using the presented data, make sure to cite the associated publication and this dataset. Abstract "Stress impacts our health and triggers physiological adaptations, yet the metabolic programs engaged during stress remain incompletely understood. To fill this knowledge gap, we utilize total-body positron emission tomography (PET), multi-OMICS, and endocrine profiling to assess how various murine stress models affect systemic metabolic remodeling. We find that acute immobilization and surgery activate brown adipose tissue as part of the stress response, independently of hypothermia, thereby acting as a highly stress-sensitive metabolic hub. Additionally, we identify stress-specific hypo- and hypermetabolic signatures in different brain regions, and distinguish networks between brain and adipose tissue depots across the different stress groups. Our work presents a novel perspective on stress and its mobilization of metabolic resources and identifies PET imaging of brain and adipose tissue as a valuable, minimally invasive technique for tracking metabolic stress responses in mice, with relevance for animal welfare and disease models, and translational impact for mental health studies and preventive medicine." Repository Structure metadata file 5 main figure source data 13 supplementary figure source data additional data (qPCR, RNASeq, Proteomics, IHC) code (zip) Additional Notes Experimental groups, sample identifiers, and measurement units are provided in the respective data files. For details regarding experimental procedures, data processing, normalization, and statistical analyses, please refer to the methods section of the associated publication. Missing values, excluded samples, and other data-processing decisions should be interpreted according to the information provided in the corresponding files and manuscript. Raw sequencing reads are deposited separately in the European Nucleotide Archive (ENA). ENA study accession: PRJEB124202. Raw Proteomics data are deposited separately in the PRIDE Repository. PRIDE accession number: PXD082804. For any further questions, please contact the corresponding author.