Gene expression in chicken wing flight feather regeneration

Avian feather has robust regeneration capability. Long noncoding RNAs (lncRNAs) are non-protein coding transcripts that are involved in various biological processes. We infected the regenerating feather follicles with lentivirus to over express Wnt5a or knock down two lncRNAs (lnc3501&lnc7831) separately. The regenerated follicles were dissected at 4 days after infection. We compared gene expression of infected follicles with normal T4 control follicles. Our results revealed that lncRNAs may modulate Wnt signaling.禽鸟类羽毛具备强大的再生能力。长链非编码RNA（long noncoding RNAs，lncRNAs）是一类不编码蛋白质的转录本，参与多种生物学过程。本研究通过慢病毒感染再生羽毛毛囊，分别开展两种处理：过表达Wnt5a，或分别敲低lnc3501与lnc7831两种长链非编码RNA。于感染后第4天解剖获取再生毛囊，并将感染组毛囊的基因表达水平与正常T4对照毛囊进行对比。研究结果表明，长链非编码RNA可能调控Wnt信号通路。