01_Transcription_levels_of_some_genes_involved_in_starch_metabolism_in_ChlreSEX4_overexpressing_algal_lines.tab

The file displays tables with the relative expression for each studied gene in different algal lines (WT, 13, 24, and 48), with each gene shown in different colors. The column "SAMPLE NAME" indicates which algal line is being analyzed. The column "TARGET NAME" indicates which gene is being analyzed, including its name and its ORF name. The column "CT" shows the Ct value obtained after qRT-PCR for each gene and each algal line, using the StepOnePlus Real-Time PCR System under the following conditions: 2 min denaturation at 94 °C; 40 cycles at 94 °C for 10 s, 60 °C for 15 s, and 72 °C for 20 s, followed by 10 min extension at 72 °C. Three replicates were performed for each sample and each gene. The column "AVERAGE CT" shows the average Ct obtained for the EEF1A gene in each line, which is used as an internal control. The column "DELTA CT" is the difference between the Ct for the specific gene under study and the average Ct for the EEF1A gene in each algal line. The column "2^-DELTA CT" is the result of calculating 2^-DELTA CT for each gene in each line. The column "average exp" is the average 2^-DELTA CT value obtained for each gene for each algal line. The column "relative expression" is the individual value of 2^-DELTA CT obtained for each gene and each line after considering the average 2^-DELTA CT for that gene in the WT line as 1.本文件展示了不同藻类品系：野生型（WT）、13号、24号及48号品系中各研究基因相对表达量的表格，各基因以不同颜色区分呈现。列"SAMPLE NAME"（样本名称）用于标注当前分析的藻类品系。列"TARGET NAME"（靶标基因名称）用于标注当前分析的基因，包含其通用命名及开放阅读框（Open Reading Frame, ORF）名称。列"CT"（循环阈值，Cycle Threshold, Ct）展示了采用StepOnePlus实时荧光定量PCR系统（StepOnePlus Real-Time PCR System），按以下反应条件完成实时定量逆转录聚合酶链反应（quantitative Reverse Transcription Polymerase Chain Reaction, qRT-PCR）后，各基因与各藻类品系对应的Ct值：94℃变性2 min；94℃下10 s、60℃下15 s、72℃下20 s循环40次，随后72℃延伸10 min。每个样本及每个基因均进行3次重复实验。列"AVERAGE CT"（平均Ct值）展示了各藻类品系中作为内参基因的EEF1A基因的平均Ct值。列"DELTA CT"（ΔCt值）为对应藻类品系中，待研究基因的Ct值与该品系内EEF1A基因平均Ct值的差值。列"2^-DELTA CT"（2^(-ΔCt)）为各藻类品系中各基因的2的负ΔCt次方计算结果。列"average exp"（平均表达量）为各藻类品系中各基因的平均2^(-ΔCt)值。列"relative expression"（相对表达量）指以野生型品系中对应基因的平均2^(-ΔCt)值为基准（设为1），计算得到的各藻类品系中各基因的单样本2^(-ΔCt)值。