Reciprocal Best Structure Hits

In this work, we are using AlphaFold structure models to find the closest homologues proteins between Homo sapiens and D. melanogaster, C. elegans, S. cerevisiae and S. pombe as well as between S. cerevisiae and S. pombe. We are using the structure aligner Foldseek to run all against all and search for the best scoring hit in both directions to detect the Reciprocal Best Structure Hits (RBSH). We compare the results to protein pairs detected by their sequence similarity as Reciprocal Best Hits (RBH) and verify the results using the PANTHER family classification files. $$ \ $$ Note: This dataset is an earlier version of a more up-to-date dataset at https://doi.org/10.17863/CAM.87873

本研究采用AlphaFold结构模型（AlphaFold structure models），在智人（Homo sapiens）与黑腹果蝇（D. melanogaster）、秀丽隐杆线虫（C. elegans）、酿酒酵母（S. cerevisiae）以及粟酒裂殖酵母（S. pombe）之间，同时在酿酒酵母与粟酒裂殖酵母之间，搜寻亲缘关系最近的同源蛋白。本研究使用结构比对工具Foldseek开展全对全比对，并在双向场景下搜索得分最高的匹配结果，以识别双向最佳结构匹配（Reciprocal Best Structure Hits，RBSH）。我们将上述结果与基于序列相似性检测得到的双向最佳匹配（Reciprocal Best Hits，RBH）进行对比，并借助PANTHER家族分类文件（PANTHER family classification files）对结果予以验证。

注：本数据集为https://doi.org/10.17863/CAM.87873所收录的最新版数据集的早期版本。