Brain transcriptome profiles in mouse model simulating features of post-traumatic stress disorder

Social stress mouse models were used to simulate human post-traumatic stress disorder (PTSD). C57B/6 mice exposed to SJL aggressor mice exhibited behaviors accepted as PTSD-in-mouse phenotype: 'frozen' motion, aggressor's barrier avoidance, startled jumping, and retarded locomotion. Transcripts in hippocampus, amygdala, medial prefrontal cortex, ventral striatum (nucleus acumbens), septal region, corpus striatum, hemi-brain, blood, spleen and heart of stressed and control C57B/6 mice were analyzed using Agilent's mouse genome-wide arrays. C57B6 mice were exposed to SJL aggressor mice for periods of 5 days and 10days (6 hours each day) to induce anxiety/stress which parallels to PTSD in human Organs, blood and brain regions were collected after 24 hours and 1.5 week of post 5 days social defeat period; and 24 hour and 6 weeks post 10 days social stress period.本研究采用社交应激小鼠模型模拟人类创伤后应激障碍（PTSD）。将C57B/6小鼠暴露于SJL攻击小鼠后，其表现出被公认为小鼠创伤后应激障碍表型的行为：僵直不动、回避攻击小鼠所在的隔离区域、受惊跳跃以及运动迟缓。本研究采用安捷伦（Agilent）小鼠全基因组表达芯片，对应激组与对照组C57B/6小鼠的海马体（hippocampus）、杏仁核（amygdala）、内侧前额叶皮层（medial prefrontal cortex）、腹侧纹状体（ventral striatum，nucleus acumbens）、隔区（septal region）、纹状体（corpus striatum）、半脑（hemi-brain）、血液、脾脏及心脏中的转录本进行了分析。实验中将C57B/6小鼠与SJL攻击小鼠分别共处5天和10天（每日6小时），以诱导出与人类创伤后应激障碍相似的焦虑与应激状态。在5天社交挫败造模结束后的24小时及1.5周时，采集器官、血液及脑区样本；在10天社交应激造模结束后的24小时及6周时，采集对应样本。