The epitope sequence is from the reference cited: Nisbet et al. (1981) Eur J Biochem 115: 335-345 [PMID: 7016535]. The authors report an Arg residue instead of a Lys at position 9, determined by micro
All but one of the hybridomas were from CD-1 mice. The exception, JAR 65, was from a human fH transgenic BALB/c mouse [43]. *fHbp mutants with amino acid substitutions at these positions lost (knocked
aPurified recombinant WNV-NS1 protein and DENV-NS1 were used as the coating antigen and were reacted with purified MAbs against the NS1 protein. The absorbance was measured at 450 nm: +, OD450 = 0.5 t