Endothelial cells mediated by STING signaling regulate oligodendrogenesis and myelination during brain development

To gain further insight into how endothelial STING regulates oligodendrogenesis, RNA-seq was used to analyze the genome-wide changes resulting from isolated endothelial cells of E18 endothelial STING conditional knock out mice and littermate wild-type.mRNA from E18 isolated endothelial cells of wild-type (WT) and STINGf/f;Tie2-cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics mRNA profiles of E18 wild type (WT) and STINGECKO mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.为深入解析内皮STING（endothelial STING）调控少突胶质细胞生成（oligodendrogenesis）的分子机制，本研究采用RNA测序（RNA-seq）技术，对E18胎龄内皮STING条件性敲除小鼠及其同窝野生型小鼠的分离内皮细胞进行全基因组表达变化分析。本研究提取了野生型（WT）与STINGf/f;Tie2-cre小鼠E18胎龄分离内皮细胞的总mRNA；具体实验流程如下：首先采用Agilent 2100生物分析仪（Agilent 2100 Bioanalyzer）对总mRNA进行质量控制与定量，随后将mRNA反转录为cDNA并构建测序文库。本次测序由安诺优达基因科技（Annoroad Genomics）依托Illumina HiSeq 2500平台完成。E18胎龄野生型（WT）与STING内皮条件性敲除（STINGECKO）小鼠的mRNA表达谱通过Illumina HiSeq 2500平台完成三次重复深度测序得以构建。