Endothelial cells mediated by STING signaling regulate oligodendrogenesis and myelination during brain development

To gain futher insight into how endothelial STING regulates oligodendrogenesis ,RNA-seq was used to analyze the genome-wide changes resulting from isolated endothelial cells of E18 endothelial STING conditional knock out mice and littermate wild-type.mRNA from E18 isolated endothelial cells of wild-type(WT) and STINGf/f;Tie2-cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics Overall design: mRNA profiles of E18 wild type (WT) and STINGECKO mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.