Overexpression of α-SMA led to differential gene expression in INS-1 cells (RNA-seq)

The goal of this study was to define the cohort of genes whose expression is regulated by α-SMA function in type 2 diabetic mellitus . Towards this goal, INS-1 cells stably expressing α-SMA (INS-SMA) were established. In addition, a control cells (INS-MOCK) was generated by transfection with empty vector. Subsequently, total RNA was isolated and then performed transcriptome RNA sequence.本研究旨在明确2型糖尿病（type 2 diabetic mellitus）中受α-SMA（α平滑肌肌动蛋白）功能调控的基因集合。为此，本研究构建了稳定表达α-SMA的INS-1细胞株（命名为INS-SMA）；此外，通过转染空载体构建了对照细胞株INS-MOCK。随后提取总RNA并开展转录组RNA测序。