Whole transcriptome analysis of neuroblastoma cell lines after ERK5 silencing

The transcriptomic network modulated after the depletion of the tyrosin kinase ERK5 through shRNA-mediated silencing was analyzed in SK-N-BE(2) and CHLA-90 neuroblastoma cell lines, in order to characterize and reveal any relevant role of this kinase at the transcriptional level. SK-N-BE(2) and CHLA-90 cells were transduced with lentiviral particles carrying two different shRNA for ERK5 silencing and 72 h later RNA was extracted in biological triplicates for expression microarray analysis.