Discover – MB Gene Expression

Bulk RNA sequencing was performed on medulloblastoma samples collected from Ptch1⁺/⁻ mice following neonatal γ irradiation (0 Gy, 0.1 Gy, 2 Gy). Libraries were generated from total RNA extracted from freshly dissected tumors. Sequencing was carried out on an Illumina platform, producing paired end reads (150 bp). Samples included tumors arising spontaneously (0 Gy) or following low dose (0.1 Gy) or high dose (2 Gy) irradiation, with metadata specifying irradiation dose, latency, sex, and experimental batch. The dataset includes raw FASTQ files, and associated sample information, enabling downstream differential expression analyses and integrative comparisons across dose groups.