Senohemostatic role for DPP4 in atherosclerosis

We performed scRNA sequencing to understand the composition and differential gene expression changes in aortic cells from LDLR-/- mice fed a normal diet (ND), high-fat diet (HFD), or HFD + DPP4i. We combined this technique with CITE-sequencing to measure the cell surface protein expression (DPP4) and transcriptomic changes associated with our experimental conditions as well as changes associated with DPP4 cell surface expression in all aortic cells.本研究针对喂食正常饮食（ND）、高脂饮食（HFD）或高脂饮食联合二肽基肽酶4抑制剂（DPP4i）的LDLR基因敲除（LDLR-/-）小鼠的主动脉细胞，开展单细胞RNA测序（scRNA sequencing），以解析其细胞组成及差异基因表达变化。本研究将该测序技术与CITE测序（CITE-sequencing）相结合，用于检测细胞表面二肽基肽酶4（DPP4）的表达水平，同时分析与本实验条件相关的转录组变化，以及所有主动脉细胞中与DPP4细胞表面表达相关的变化。