Gene expression of blood and cerebellum of Mecp2-null and WT male mice

In order to find a relationship between gene expression of blood and brain in Rett Syndrome (RTT), we performed RNA sequencing on from cerebella and blood of 7 week-old male Mecp2-null mice (a model of RTT) and WT controls. Transcriptional profiles were generated from cerebellum and blood of 3 Mecp2-null and 3 WT 7 week-old male mice, by RNAseq performed on an Illumina HiSeq 2000 System, generating approximately 60 million 2x75bp paired-end reads/sample. Blood and cerebellum samples originate from the same animal为探究雷特综合征（Rett Syndrome, RTT）的血液与大脑基因表达关联，我们对7周龄雄性Mecp2基因敲除小鼠（Mecp2-null mice，RTT疾病模型）及野生型（Wild Type, WT）对照小鼠的小脑与血液组织开展RNA测序实验。本研究共纳入3只Mecp2基因敲除小鼠与3只同周龄雄性野生型小鼠，采集其小脑及血液样本，采用Illumina HiSeq 2000测序系统进行RNAseq测序，每个样本可生成约6000万条2×75bp双端读段（paired-end reads）。所有血液与小脑组织样本均取自同一只受试小鼠。