Ectopic misexpression of Irx3 and Irx5 in the paraventricular nucleus of the hypothalamus contributes to hyperphagia and neurodevelopmental defects in Sim1 haploinsufficiency

Single cell sequencing of Sim1-Cre;tdTomato+ FACS sorted cells from hypothalami of wildtype and Sim1 heterozygous mice. Single-cell RNA sequencing (Drop-Seq) was performed on tdTomato+ FACS sorted cells hypohalami of Sim1-Cre;tdTomato and Sim1-Cre;tdTomato;Sim1Het (double-heterozygous) mice at postnatal day 1 to 4. For each genotype, hypothalamus tissues from 6 pups were pooled for single-cell dissociation, followed by FACS sorting and single-cell RNA sequencing.本数据集为针对野生型与Sim1杂合子小鼠下丘脑来源的、经荧光激活细胞分选（FACS, fluorescence-activated cell sorting）获得的Sim1-Cre;tdTomato阳性细胞的单细胞测序数据。研究人员对出生后1至4日龄的Sim1-Cre;tdTomato以及Sim1-Cre;tdTomato;Sim1Het（双杂合）小鼠的tdTomato阳性FACS分选细胞开展了单细胞RNA测序（Drop-Seq）。针对每种基因型，研究人员将6只幼鼠的下丘脑组织混合后进行单细胞解离，继而完成FACS分选与单细胞RNA测序。