Association (Kon) and dissociation (Koff) rate constants for purified MAb DB81 were measured by surface plasmon resonance. The Kd was calculated as Koff\\Kon.
Thirty-two mAbs are included for this comparison and are ranked by their MSD/SPR affinity correlation (best to worst). ND = not determined; NB = non-binder in the assay; Bold = kd-limited in the Biaco
*IC50 values reflect concentration of Tfab required to compete 50% of saturating Tmab IgG. Errors are standard deviations from technical triplicates. In vitro binding affinity of Tfab and Tmab.
