Supplement 3. sgRNA sequences used for second round screen. from CRISPR Genome-Wide Screening Identifies Dependence on the Proteasome Subunit PSMC6 for Bortezomib Sensitivity in Multiple Myeloma

The 31 most abundant of these sgRNAs found in cells surviving BTZ treatment were selected for secondary screening (Figure 2B). Each of the 31 potential BTZ resistance inducing genes was targeted by four sgRNAs, including three sgRNAs from the GeCKO V2 library A and the fourth from GeCKO V2 library B.