Single-nucleus transcriptome profiling of rat liver under CIH

Obstructive sleep apnea (OSA) has been demonstrated to be associated with liver injury. However, the cellular and molecular mechanisms by which chronic intermittent hypoxia (CIH), a hallmark of OSA, contributes to liver injury remain poorly understood. Twelve male Sprague-Dawley rats were randomized into normoxic control (NC) and CIH groups (n = 6 each), with CIH exposure for 12 weeks. liver injury was evaluated by Hematoxylin and Eosin staining. Single-nucleus RNA sequencing (snRNA-seq) was performed. Histopathological analysis revealed liver injury withcell edema, necrosis in the CIH group compared to NC.