RNaseH2B single and compound mutant cerebellum

We utilized RNA-seq analysis to examine the loss of RNaseH2B in the mouse cerebellum over time. We have looked at single and compound mutant knockouts at ages from postnatal day five, postnatal day twenty-eight and nine-week-old animals. RnaseH2B mutant RNA-seq total stranded from 9 week and p5, poly-A enriched for age p28.本研究采用RNA测序（RNA-seq）技术，分析了核糖核酸酶H2B（RNaseH2B）在小鼠小脑中的随时间缺失情况。我们选取出生后第5天、出生后第28天以及9周龄的小鼠，对单突变体与复合突变体敲除模型进行检测。其中，针对9周龄和出生后第5天的RNaseH2B突变体样本，采用链特异性总RNA测序方案；针对出生后第28天的样本，则采用polyA富集型测序方案。