Diurnal oscillations in murine brown adipose tissue analyzed by RNA-seq

Brown adipose tissue (BAT) burns fatty acids to produce heat, and shows diurnal oscillation with peak activity in glucose and triglyceride-derived fatty acid uptake at wakening. To gain further insight in the diurnal regulation of metabolic BAT activity, we performed RNA-sequencing (RNA-seq) on interscapular (i)BAT samples collected from chow-fed male C57BL/6J mice (10 weeks old) exposed to mild cold (22°C) at 3-hour intervals throughout a 24-hour period (n=4 mice per time point). All mice were entrained to a 12h:12h light:dark cycle, and therefore time is denoted as Zeitgeber Time (ZT) in which ZT0 indicates the onset of the light (active) phase. We applied RNA-seq on total RNA isolated from murine iBAT obtained at 3 hour-intervals throughout a 24-hour period (8 time points in total; 4 replicates per time point).棕色脂肪组织（Brown Adipose Tissue, BAT）可通过燃烧脂肪酸产热，并呈现昼夜节律振荡，在觉醒时刻葡萄糖及甘油三酯衍生脂肪酸的摄取活性达到峰值。为深入解析代谢性棕色脂肪组织活性的昼夜调控机制，我们对24小时内每3小时采集一次的肩胛间棕色脂肪组织（interscapular BAT, iBAT）样本进行了RNA测序（RNA-sequencing, RNA-seq）。实验样本取自普通饲料喂养、处于轻度寒冷环境（22℃）的10周龄雄性C57BL/6J小鼠，每个时间点采集4只小鼠的样本（n=4/时间点）。所有小鼠均经12小时光照:12小时黑暗的光暗周期驯化，因此实验时间以授时因子时间（Zeitgeber Time, ZT）标注，其中ZT0代表光照（活动）阶段的起始时刻。我们对24小时内每3小时采集的小鼠肩胛间棕色脂肪组织总RNA进行了RNA测序，共计设置8个时间点，每个时间点包含4次生物学重复。