RNAseq analysis of mouse CD4+ regulatory Tregs in WT and deltaFoxp3 mice isolated from spleen and lungs.

RNAseq analysis of mouse CD4+ regulatory Tregs in 21-day-old male WT and deltaFoxp3 mice isolated from spleen and lungs. Mouse CD4+ regulatory Tregs in 21-day-old male WT and deltaFoxp3 mice were isolated from spleen and lungs by flow cytometry, as DAPI-TCRbeta+CD4+GFP+CD25+. 1,000 cells were double-sorted directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org).本数据集针对21日龄雄性野生型（Wild Type, WT）及deltaFoxp3小鼠脾脏与肺脏中分离的CD4+调节性T细胞（regulatory T cells, Tregs）开展RNA测序（RNA-seq）分析。实验采用流式细胞术，以DAPI⁻TCRβ⁺CD4⁺GFP⁺CD25⁺为分选标记，从上述21日龄雄性WT及deltaFoxp3小鼠的脾脏与肺脏中分离CD4+调节性T细胞；随后将1000个细胞直接进行双分选并加入裂解缓冲液，参照ImmGen官方公开的标准低起始量实验方案（www.immgen.org），采用Smart-seq2技术完成RNA测序文库构建。