RNA-seq analysis of WT and betaOGT KO female islets

We report our results from two runs of RNA-seq analysis on islets isolated from female C57Bl/6J mice with a constitutive deletion of O-GlcNAc Transferase (OGT) specifically in pancreatic beta-cells (Rip-cre; Ogt f/f) and controls (Ogt f/f or Ogt f/+) RNA was isolated from snap-frozen islet pellets. Libraries were created using standard Illumina reagents and analyzed using a HiSeq2500.本研究报道了两次RNA测序（RNA-seq）分析的结果，分析对象为雌性C57Bl/6J小鼠的胰岛：其中一组小鼠在胰腺β细胞中特异性存在O-连接β-N-乙酰葡糖胺转移酶（O-GlcNAc Transferase，OGT）的组成型敲除，基因型为Rip-cre; Ogt f/f；对照组小鼠基因型为Ogt f/f或Ogt f/+。实验从快速冷冻的胰岛沉淀中提取总RNA，采用标准Illumina试剂构建测序文库，并通过HiSeq2500平台完成测序分析。