RNA-seq of WT, hrr25as, and hrr25is treated with inhibitors

The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcrption. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific readers. While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the transcriptome in WT cells, hrr25as cells, or hrr25is cells in the presence or absence of ATP analog inhibitors.RNA聚合酶II（RNA polymerase II, Pol II）最大亚基的羧基末端结构域（carboxyl-terminal domain, CTD）可协调转录不同阶段中特定细胞机器的动态招募。该CTD的Y1S2P3T4S5P6S7七肽重复序列的特征性磷酸化模式，可招募特异性阅读器蛋白。尽管磷酸化丝氨酸5（phospho-Ser5）与磷酸化丝氨酸2（phospho-Ser2）标记广泛存在，但已有研究显示磷酸化苏氨酸4（phospho-Thr4）仅对特定基因发挥调控作用。本研究对野生型（WT）细胞、hrr25as细胞以及hrr25is细胞在ATP类似物抑制剂存在与否条件下的转录组进行了探究。