ChIP-chip in Hrr25 mutant strains

The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcrption. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific readers. While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the genome-wide occupancy of Pol II, phospho-Thr4, Hrr25, and key reader Rtt103 in WT and irreversibly sensitive Hrr25 (hrr25is) mutant strains of S. cerevisiae.RNA聚合酶II（RNA polymerase II，Pol II）最大亚基的羧基末端结构域（carboxyl-terminal domain，CTD）可在转录的不同阶段协调特定细胞分子机器的动态招募。CTD的Y1S2P3T4S5P6S7七肽重复序列的特征性磷酸化模式，可结合特定的读取因子。尽管磷酸化Ser5与磷酸化Ser2标记普遍存在，但据报道磷酸化Thr4仅对特定基因发挥调控作用。本研究针对酿酒酵母（Saccharomyces cerevisiae，S. cerevisiae）的野生型（wild type，WT）及不可逆敏感型Hrr25（hrr25is）突变菌株，分析了Pol II、磷酸化Thr4、Hrr25以及关键读取因子Rtt103的全基因组结合占据情况。