RNAseq of mitral valves from Fbn1 C1039G/+ mice with or without CCR2 deficiency

Mitral valves were isolated from 2-month-old mice with the following 4 genotypes: 1) CCR2 RFP/+ (Chet), 2) CCR2 RFP/RFP (Cko), 3) Fbn1 C1039G/+; CCR2 RFP/+ (FhetChet), 4) Fbn1 C1039G/+; CCR2 RFP/RFP (FhetCko). N=4 for each genotype. Total RNA was extracted from mitral valves individually for gene expression profiling. Total RNA from mitral valves of each mouse was used for deep RNA sequencing individually本研究从4种基因型的2月龄小鼠中分离二尖瓣，其基因型分别为：1）CCR2^RFP/+（Chet）；2）CCR2^RFP/RFP（Cko）；3）Fbn1^C1039G/+; CCR2^RFP/+（FhetChet）；4）Fbn1^C1039G/+; CCR2^RFP/RFP（FhetCko）。每种基因型的样本量为4。随后，分别从每枚二尖瓣中提取总RNA以进行基因表达谱分析；每只小鼠的二尖瓣总RNA均单独用于深度RNA测序。