Effects of depletion of Rho-associated, coiled-coil-containing protein kinase 2 in the kidney medullary tissue of murine model.

Rho and its effector Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) play crucial roles in a variety of cellular functions, such as cell adhesion and cytoskeletal rearrangement . We here investigated the role of ROCK2 in the kidney medullary tissue using tubular specific ROCK2 knockout mice. To investigate the role of ROCK2 in kidney tubular cells, we established the tubular specific ROCK2 knockout mice. RNA-seq on the kidney medullary tissue.Rho蛋白及其效应蛋白Rho相关卷曲螺旋包含蛋白激酶2（Rho-associated, coiled-coil containing protein kinase 2，ROCK2）在多种细胞生命活动中发挥关键调控作用，包括细胞黏附与细胞骨架重排。为探究ROCK2在肾小管细胞中的生物学功能，本研究构建了肾小管特异性ROCK2敲除小鼠模型，并利用该模型分析ROCK2在肾髓质组织中的作用；随后对该模型小鼠的肾髓质组织进行了RNA测序（RNA-seq）。