These sequences were checked against the following quality criteria: (i) no Ns, (ii) quality score ≥ 23 according to the PANGEA process (Giongo et al., 2010), (iii) a minimum sequence length of 200 bp
To verify some of the significant CpGs from the genome-wide analysis, we selected the target CpGs at the warfarin-associated genes. Thereafter, we used pyrosequencing to detect the methylation
De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for glyceraldehyde-3-phosphate dehydrogenase (GAPDH
