All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for either 1, 2, 4, 8, 16 or 48 hours. Test total RNA was ex
To unravel the molecular events caused by exposure to dermal fillers in a multi-cell type human 3D skin model and to link these events to the characteristics of the dermal fill-ers. The human Phenion®
gmMφ and mMφ were stimulated with ICs (50 µg/ml) (A), LPS (100 ng/ml) or LPS+ICs (B) and TNFα and IL-10 were measured in culture supernatants after 20 hours. (C) gmMφ were stimulated with Pam3CSK4 (5
Most cell culture experiments utilize media containing fetal calf serum. Results are often interpreted regarding importance to human pathways. We studied gene expression in mouse macrophages grown in